Microstructural evidence of reversal of PCOS by steroidal saponins of asparagus racemosus in PCOS induced rats

The purpose of this study is to investigate the histological and hormonal observations in fructose-fed, letrozole-induced polycysticovarian- syndrome (PCOS) rats treated with various doses extract asparagus racemosus (EAR) Steroidal saponin (SAPO). 48 female Wistar albino were divided into 8 groups, including Vehicle Control (VC); PCOS; EAR 400 mg/kg; SAPO 40 PCOS + 200 20 mg/ kg; mg/kg. group was administered letrozole at a concentration 1 mg/kg dissolved 1% CMC per oral(p.o.) once daily for 28 days. Along these, allowed free access 10% fructose solution daily. Calculated dosages given oral gavage 30 During experimental period, vaginal smears collected estrus cycle determination. Rats sacrificed blood samples assay. Ovaries removed proceed histopathological study. Slides stained using hematoxylin eosin (H&E) stains. When compared vehicle control group, ovaries had higher incidence ovarian cysts, incomplete luteinization, lower number corpus lutea. Although serum estradiol, progesterone, Follicle-stimulating hormone (FSH) levels testosterone luteinizing (LH) higher. findings indicated that taking 40mg/kg orally could alleviate PCOS-related symptoms. It appears consuming reduces LH while increasing FSH, estrogen, progesterone levels. Because balancing nature these drugs, 400mg/kg- 40mg/ kg-treated cystic follicles corpora In rats, results normal process folliculogenesis ovulation. current study, we observed better 400mg/kg treatment.